Anti-aging; HRW is an anti- aging agent thatincreases telomere length & improves DNA methylation.. It also improves sleep quality and functioning in the elderly.
Author
Zanini, et al.
Treatment
Duration (days)
180
Year Published
2021
Dose
7.5 mg H2; HRW (15ppm of hydro- gen) two time daily in the morning & the evening on empty stomach for 6 months.
Inclusion
Criteria
1. Age: 70 and over.
2. Body mass index normal or overweight range.
3. No current acute disorders or major chronic diseases (e.g., cancer, neurodegenerative disorders, stroke, psychiatric diseases).
4. Able to read and understand written consent form..
5. Willing to take blood tests and other measurements.
6. Willing to conusme assigned intervention.
Number of
Subjects
40
Outcome
(positive)
1. Significant treatment vs time interaction for telomere length (p<0.05). Treatment group increased telomere length (from 0.99 ± 0.15 to 1.02 ± 0.26, up 4.1% at 6-month follow up), whereas the control group lost telomere length (from 0.92 ± 0.27 to 0.79 ± 0.15, down 11.1% at 6-month follow-up).
2. Brain choline and n-acetylaspartate levels increased in the left frontal gray matter, and creatine at the right parietal white matter. Brain n-acetylaspartate at the right parietal mesial grey matter (p<0.05)
3. Human growth hormone and serum IGF-1 levels tended to rise after HRW intervention.
4. LDL tended to decrease in HRW group, while triglycerides reduced in both groups
5. Serum malondialdehyde levels and ferric reducing ability of plasma strongly tended to decrease after HRW intervention (for 48.7% and 8.4%, respectively).
6. A significant change in improved chair stand performance in HRW group.
7. Quality of sleep improved in HRW group.
8. Significant interaction for pain domain of the quality of life profile with HRW vs placebo (p<0.05). VAS pain score
9. TET2 expression increased in both groups, but significantly more in HRW group (0.81 ± 0.52 at baseline to 1.13 ± 0.82)
10. All oxidative stress and inflammation markers tended to decrease.
11. Serum magnesium levels tended to increase in both groups, although marginally significantly more in the HRW group"
Pre-hypertension, metabolic syndrome, central obesity; HRW reduced cholesterol, glucose, HbA1c, and markers of inflammation and oxidative stress. Mildly reduced body mass index and waist to hip ratio.
Author
LeBaron, et al.
Treatment
Duration (days)
168
Year Published
2020
Dose
11 mg H2/day;1 tablet 3 x daily in 250 mL of 12-18°C water, producing >5.5 mmol
Inclusion
Criteria
prehypertension/hypertension (systolic blood pressure [BP] > 130 mmHg and/or diastolic BP > 85 mmHg), prediabetes/diabetes (fasting glucose > 110 mg/dL), central obesity (waist circumference [WC] > 90 cm for men, and WC > 80 cm for women), and dyslipidemia (high-density lipoprotein [HDL] < 40 mg/dL for men and < 50 mg/dL for women; triglycerides [TG] > 200 mg/dL)
Number of
Subjects
60
Outcome
(positive)
11 mg H2/day;1 tablet 3 x daily in 250 mL of 12-18°C water, producing >5.5 mmol
All oxidative stress markers (TBARS, melonaldehyde, and diene conjugates) changed in the favorable direction (P < 0.05), although TBARS was not statistically significant
2. Significantly reduced inflammation based on blood CRP, TNF-alpha, and IL-6
Vitamin E & C significantly increased in HRW group compared to control
4. reduction in total cholesterol by 18.5 mg/dL (P < 0.05).
5. triglycerides levels by ~ 47 mg/dL (P < 0.05).
6. Fasting blood glucose decreased from 121.5 ± 61.0 to 103.1 ± 33.0 mg/dL
7. 12% reduction in HbA1c (P < 0.05). 8. Reduced heart rate, body mass index, and waist-hip circumference (P < 0.05).
8. Reduced heart rate, body mass index, and waist-hip circumference (P < 0.05).
9. Nitrites increased more in the HRW group (baseline 0.63 ± 0.06 to 0.68 ± 0.06 at 24 wk) vs control (0.66 ± 0.04 to 0.65 ± 0.03)"
Sleep-deprivation relief; Improves alertness and executive function in sleep-deprived people with persistent results, while caffeine's executive function improvement dropped after 15 min. Hydrogen tablets might be superior to caffeine for fatigue and cognitive deficits from sleep deprivation.
Author
Zanini, et al.
Treatment
Duration (days)
1
Year Published
2020
Dose
4 mg of H2; A single dose with two hydrogen tablets in 500 mL water given to participants in the morning.
Inclusion
Criteria
age: 18 to 30 years.
body mass index: 18.5 to 25.0 kg/m2.
no major chronic diseases or acute disorders.
sleep deprivation of 24h
Number of
Subjects
23
Outcome
(positive)
4 mg of H2; A single dose with two hydrogen tablets in 500 mL water given to participants in the morning.
Increase in VAS alertness (point change = 1.7; 95% CI from 0.6 to 2.8; P = 0.003)
Positively impacted orientation Attention Network Test (ANT) scores from –35.8 to 40.6; P = 0.05)
HRW is comparable to caffeine for all evaluated alertness outcomes (p<0.05)
Healthy habitual coffee drinker with healthy BMI and no chronic disease, no supplement use in the past 4 weeks
Number of
Subjects
8 men and 8 women
Outcome
(positive)
4 mg H2 and/or 50-mg caffeine;
HRW and HRW + Caffeine group took less time to complete a trail-making test than the control group
HRW or caffeine group made less mistakes in the symbol digit modalities test
Both HRW and caffeine increased choline/creatinine ratios in frontal bilateral white and gray matter; HRW elevated this ratio in the paracentral brain. So, HRW had a more robust increase in metabolism in the paracentral region of the brain.
HRW and HRW + caffeine changed brain metabolism
Single digit modality test errors significantly reduced in HRW or caffeine, but not HRW + caffeine group
HRW+caffeine equivalent or better than individual components for several alertness and brain metabolism indices p<0.05 for all findings
>11 mg H2/day; 1 tablets 3x daily in 250 mL water @12 - 18oC water
Inclusion
Criteria
Indian ethnicity
Metabolic syndrome with: systolic BP>130 mmHG, diastolic BP > 85 mmHG fasting glucose >110 mg/dL central obesity dyslipidemia (HDL<40 mg/dL for women, < 50 mg/dL for men) triglycerides > 200 mg/dL
Number of
Subjects
60 subjects
Outcome
(positive)
>11 mg H2/day; 1 tablets 3x daily in 250 mL water @12 - 18oC water
Body mass index significantly reduced from baseline,, on average by 0.7 km/m^2
Waist-hip circumference ratio decreased slightly in HRW group compared to placebo, although p<0.05
Total cholesterol decreased by~27.5 mg/dL on average in the HRW group, no change in placebo group, with the main cholesterols going down being vLDL and LDL
Triglycerides dropped by ~47.4 mg/dL on average
Fasting glucose dropped from 121.5 +/- 61.0 to 103.1+/- 33.0 whereas it went up slightly in the placebo group
Inflammation markers such as TNF-alpha and IL-6 decreased
All other oxidative stress markers decreased, while antioxidants such as vitamins E and C decreased
All other oxidative stress markers decreased, while antioxidants such as vitamins E and C decreased
Pain, swelling, ankle sprain recovery; 30 min ankle bathing every 4 h significantly relieved ankle sprain pain and swelling, and improved range of motion in 24 h.
Author
Javorac, et al.
Treatment
Duration (days)
1
Year Published
2020
Dose
8 ppm H2;
1) HRW was produced by putting a magnesium-producing formulation into a 3-L stationary whirlpool with
tap water of neutral temperature (20°C).
2) They received six 30-min ankle baths with HRW throughout the first 24 h post-injury, with hydrotherapy administered every 4 hours during the intervention period.
Inclusion
Criteria
Healthy athelete with no major chronic diseases or acute disorders, and with no history of a previous AAS during the past 6 months
Number of
Subjects
18 healthy male
Outcome
(positive)
4 mg of H2; A single dose with two hydrogen tablets in 500 mL water given to participants in the morning.
Pain at rest and during movement decreased to 29.9 ± 8.1 and 44.7 ± 14.9 respectively.
Weight bearing lunge test (WBLT) increased from 4.1 ± 1.7 to 6.5 ± 1.9.
Single-leg balance with eyes open and closed significantly improved for 18.4 sec.
Interleukin-1 beta reduced from 0.6 ± 0.4 to 0.5 ± 0.3.
Resistance training muscle damage and soreness; HRW can reduce muscle damage, and ease sore muscles after high-intensity eccentric exercise in active healthy men.
Author
Todorovic, et al.
Treatment
Duration (days)
1
Year Published
2020
Dose
8 ppm H2; A single session with super saturated HRW (8mg/L of H2) for 30 mins.
Inclusion
Criteria
age from 18 to 30 years.
healthy body mass index (e.g., 18.5 to 24.9 kg/m2).
No chronic disease or acute muscloskeletal disorders.
Number of
Subjects
6 Men
Outcome
(positive)
8 ppm H2; A single session with super saturated HRW (8mg/L of H2) for 30 mins.
Change in biomarkers of exercise induced mascular damage elevation after 24 h of follow-up placebo vs HRW: creatine kinase (CK) 465±295 vs 357 ± 189 U/L, Aspartate transaminase (AST) vs 32.3 ± 7.8 to 30.0 ± 8.0 U/L between placebo and HRW groups.
Visual Analogue Scale (VAS) decreased in HRW group after bathing (32.7 ± 8.6% vs. 20.0 ± 12.8%; P=0.02) and at 24-hour follow-up (31.6 ± 24.3% vs. 22.4 ± 27.5%; P=0.03).
HRW may improve gut inflammation, improve the gut flora towards ones that produce more SCFA. The SCFA acts in the brain and mitochondria to help with body composition, metabolic health, and waist circumference in middle-aged overweight adults. N-acetylaspartate, a marker of neuronal functions, also went up in several brain regions.
Author
Korovljev et al
Treatment
Duration (days)
84
Year Published
2023
Dose
7.5 mg/day, divided into 2 doses
Inclusion
Criteria
age 18 - 65 years old apparently healthy adults
BMI > 24.9 kg/m2
Informed consent
No major chronic diseases or injuries
No other supplement use 28 days before the trial
Not participating in other trials
Number of
Subjects
10 men, 10 women, although study ended with 9 men and 9 women
Outcome
(positive)
7.5 mg/day, divided into 2 doses
Several significant reductions in body composition measures (P<0.05), i.e. in waist circumference and middle upper arm circumference
Weight, BMI, and % body fat tended towards reduction
Mild but significant reduction in triglycerides (P=0.01), while glucose tended to decrease (P=0.08)
Ghrelin significantly went up in the HRW group (P=0.02)
Fecal calprotectin, a measure of gut inflammation, significantly decreased (P=0.04)
Choline levels significantly decreased in the posterior cingulate, wheras n-acetylaspartate went significantly up (P<=0.05)
N-acetyl aspartate also went up in the prefrontal and thalamus (P<=0.05)
Sperm quality and subfertility may improve with HRW
Author
Stager, et al.
Treatment
Duration (days)
56
Year Published
2023
Dose
15 mg/day, divided into 4 doses (freshly dissolved 1 HRW tablet in 250 mL water per dose per 6 h
Inclusion
Criteria
18 - 35 years old healthy male with sperm quality test available and informed consent. No major chronic diseases or acute injuries, any supplement use in the past 4 weeks. Not participating in other studies. At least half must have oligospermia.
Number of
Subjects
12 healthy males
Outcome
(positive)
15 mg/day, divided into 4 doses (freshly dissolved 1 HRW tablet in 250 mL water per dose per 6 h
Sperm concentration and morphology tended to increase by 12.4 M/mL and by 3.8% live cells (P <= 0.30, Cohen's effect sizes 0.36 and 0.30, respectively).
HRW superior to placebo with number of live sperm cells (P = 0.03). HRW increased the percentage of live sperm by 1.1%, compared to a 6.4% drop in control group.
HRW tended to increase sperm concentration. (P = 0.25), while both interventions tended to reduce sperm volume (P = 0.18).
HRW may help reduce appetite by changing the glutamate-GABA-glutamine cycle in the brains of overweight humans, thus helping with weight loss.
Author
Korovljev, et al.
Treatment
Duration (days)
84
Year Published
2023
Dose
7.5 mg/day
Inclusion
Criteria
Apparently healthy overweight adults, no recent history of using anti-obesity medications; free from acute injuries and major chronic diseases
Number of
Subjects
5 apparently healthy individuals (3 women, 2 men)
Outcome
(positive)
7.5 mg/day
Posterior cingulate gyrus glutamate and glutamate-plus-glutamine levls decreased from 8.60 ± 1.34 mM to 6.70 ± 0.16 mM (P = 0.01), and from 9.71 ± 1.10 mM to 7.37 ± 0.62 mM (P = 0.01), respectively.
GABA in left prefrontal white matter dropped from 0.95 ± 0.99 mM to 0.29 ± 0.43; P = 0.05
Glutathione levels in anterior cingulate gyrus dropped from 0.65 ± 0.23 mM to 0.32 ± 0.23; P < 0.01
Brain taurine increased in the posterior cingulate gyrus from 0.58 ± 0.50 mM to 1.00 ± 0.23 mM, P = 0.04
Concussion; Oral transmucosal hydrogen formulation within 15 min after the injury may reduce concussion symptom severity and improve recovery from the concussion.
Author
Javorac, et al.
Treatment
Duration (days)
1
Year Published
2019
Dose
700-mg tablet producing 80 mL of H2 administered buccally every 2 hours
Inclusion
Criteria
Suffered from a sport related concussion for 30sec, he has no history of concussion or other TBI in the past 6 months and no neurological, psychiatric or other chronic conditions.
Number of
Subjects
24 year olds male
Outcome
(positive)
700-mg tablet producing 80 mL of H2 administered buccally every 2 hours
Total Sport Concussion Assessment Tool 2 (SCAT2) scores rose from 68 to 84 points
Total number of concussion symptoms decreased from 18 to 9.
Symptom severity score dropped to from 53 to 12 (out of 135).
Single-leg stance test (SLST) balance test improved by 25%
Pain, swelling, and ankle sprin recovery; acute multi-session hydrotherapy with hydrogen-rich water might be a helpful treatment in terms of pain, swelling reduction and regaining range of motion after an ankle sprain.
Author
Javorac, et al.
Treatment
Duration (days)
1
Year Published
2020
Dose
8 ppm H2;
1) six sessions of ankle and foot hydrotherapy (e.g. 30-min at every four hours) with super-saturated hydrogen-rich water during the first 24 hours post-injury.
2) Super-saturated hydrogen-rich water was produced by putting a magnesium-producing formulation (10 g) into a 3-L stationary whirlpool with tap water of neutral temperature (20°C)
Inclusion
Criteria
football athlete who suffered a sport-related ankle sprain in April 2019.
Number of
Subjects
29 year old male
Outcome
(positive)
8 ppm H2
VAS score for pain dropped from 50 points to 20 points.
Weight-bearing lunge test (WBLT) improved from 43.1 mm to 55.0 mm.
The injured ankle swelling decreased from 56.5cm to 54.9 cm
Toe injury pain, swelling, and recovery; Soaking foot in high concentration hydrogen water can reduce pain, and improve function and recovery from a toe injury.
Author
Tarnava, A.
Treatment
Duration (days)
7
Year Published
2021
Dose
8 ppm H2; The case received four sessions (25 mins hydrogen rich hydrotherapy session) in a super saturated HRW with an average concentration 8mg/L.
Inclusion
Criteria
The case suffered from sport injury while kickboxing, resulting in a potential fracture of the proximal phalanges on the 5th toe of his right foot.
No history of any broken bone reported in the past 12 months.
Number of
Subjects
35 year old male
Outcome
(positive)
8 ppm H2;
7-day follow up improvements: lower pain, full ability of the toe to move
Visual signs of swellings and bruising were reduced.
